The goal of this guide is to provide mine operators, occupational safety and health (OSH) managers, and others with actionable tools to more effectively plan, implement, and integrate a range of workplace interventions to prevent opioid use and opioid use disorder among mine workers. It was developed collaboratively by the Mine Safety and Health Administration (MSHA) and the National Institute for Occupational Safety and Health (NIOSH). Feedback from mining community constituents, existing workplace opioid use prevention guidance, and the prevention research literature informed the design of this guide.